Abstract: In the present study, ‘Legacy’ blueberries were stored in a pre-refrigerated storage within 2 hours after harvest, and then treated with 1-methylcyclopropene（1-MCP） at concentrations of 0.5, 1.0, 1.5 μL/L in a controlled atmosphere chamber. After 96 hours of transport by cold chain truck, blueberries were stored in the ice temperature storage（（-0.5±0.3）℃）, and the quality indices, physiological indices and volatile components were determined every 10 days,in order to explore the effects of different concentrations of 1-MCP combined with modified atmosphere package（MAP） on the fresh-keeping effects of blueberries during cold storage. The results showed that MAP combined with three concentrations of 1-MCP treatments could delay the decline of fruits firmness, maintain relatively high contents of anthocyanins, VC, soluble solids and titrable acids, and inhibit the respiratory peak and ethylene production rates.SPSS principal component analysis showed that the fresh-keeping effects could be determined as follows: MAP+1.0 μL/L1-MCP＞MAP+1.5 μL/L 1-MCP＞MAP+0.5 μL/L 1-MCP＞MAP. Aldehydes（52.34%～86.13%） and terpenoids（7.26%～32.44%） were the main volatile components of blueberries during storage, and blueberry fruits treated with MAP+1.0 μL/L 1-MCP had relatively high contents of aldehydes and terpenoids. Therefore, MAP combined with1.0 μL/L 1-MCP treatment presented the best preservation effect on blueberries.