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6-苄氨基嘌呤处理对鲜切荸荠褐变及活性氧代谢的影响

Effects of 6-benzylaminopurine Treatments on Enzymatic Browning and Reactive Oxygen Species Metabolism of Fresh-Cut Water Chestnuts

  • 摘要: 为探究不同浓度6-苄氨基嘌呤(6-BA)处理对鲜切荸荠褐变及活性氧代谢的影响,以桂蹄3号新鲜荸荠为试材,采用浓度分别为100、200、400 mg/L的6-BA浸泡鲜切荸荠3 min,以纯水浸泡处理为对照(CK),测定贮藏过程中总酚、类黄酮、醌、丙二醛(MDA)和过氧化氢(H2O2)含量变化以及多酚氧化酶(PPO)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性等相关指标。结果表明:与对照相比,6-BA处理减少了总酚、类黄酮、醌类等呈色物质的积累,进而有效抑制了鲜切荸荠褐变,维持了鲜切荸荠贮藏期间的色泽,同时降低了PPO、POD和PAL等褐变相关酶活性;此外,6-BA处理抑制了鲜切荸荠中MDA和H2O2含量,提高了贮藏期间CAT和SOD活性,进而提升了鲜切荸荠在贮藏期间的品质。通过对比不同浓度6-BA处理效果可知,400 mg/L 6-BA处理在抑制鲜切荸荠褐变及活性氧代谢方面效果最好。

     

    Abstract: In the present study,the fresh-cut water chestnuts of‘Guiti NO.3’were used as experimental materials and soaked with 6-benzylaminopurine (6-BA) of 100 mg/L,200 mg/L,400 mg/L for 3 minutes,and then the contents variations of total phenols,flavonoids,quinones,malondialdehyde (MDA),hydrogen peroxide (H2O2) and activities of polyphenol oxidase (PPO),peroxidase (POD),phenylalanine ammonium-lyase (PAL),catalase (CAT),superoxide dismutase (SOD) were evaluated using pure water treatment as control (CK),aiming to investigate the effects of 6-BA treatments with different concentrations on the browning and reactive oxygen species metabolism Results showed that 6-BA treatments reduced the accumulation of total phenolics,flavonoids and quinone etc.and decreased PPO,POD and PAL activities compared with the control,resulting in effectively inhibiting browning of fresh-cut water chestnuts and maintaining the color during storage.Moreover,6-BA treatments also suppressed the contents of MDA and H2O2,while increased CAT and SOD activities,thus improving the qualities of fresh-cut water chestnuts during storage.The 6-BA treatment of 400 mg/L presented superior effects on inhibiting browning and reactive oxygen species metabolism of fresh-cut water chestnuts

     

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