Abstract: In order to study effects of different fertilization methods on postharvest hardness of kiwifruit,‘Donghong’kiwifruit was picked at physiological maturity stage and was stored at room temperature. Hardness, softening rate,content of starch, protopectin, soluble pectin, cellulose, enzyme activities of polygalacturonase（ PG）, pectin methylesterase（PME）, cellulase（Cx）,and β-galactosidase（β-Gal） were frequently sampled and analyzed. Results showed that reasonable fertilization could inhibit the hardness loss and the decrease of nutritious components（starch, protopectin and cellulose）, and delay the increase of soluble pectin and repress the enzyme activities such as PG, PME, Cx, β-Gal and amylase. Hardness of kiwifruit cultivated by different fertilization methods was negatively correlated with soluble pectin content and activities of PG, Cx, β-Gal and amylase（ P＜0.01）, and positively correlated with protopectin, cellulose and starch content（P ＜0.01）. Combination of compound chemical fertilizer,organic fertilizer and biological fungus fertilizer in kiwifruit production can significantly delay fruit softening and prolong fruit storage.