Abstract: In order to monitor the import of transgenic maize MIR162 and standardize the circulation of transgenic maize in domestic market,an accurate and fast detection method for transgenic maize MIR162 was established.By designing primers and probes for the specific sequence of transgenic maize MIR162,the multiple fluorescent PCR system and parameters were explored and optimized,and a multiple real-time fluorescent PCR with TaqMan-MGB hybridization probe labeling method for rapidly identifying transgenic maize MIR162 was developed and established.The results showed that the internal standard gene and line-specific gene of transgenic maize MIR162line standard and parallel samples presented obvious amplification curves,and only the internal standard gene of other transgenic maize line standards exhibited amplification curves,while the blank control had no amplification curves,indicating that the TaqMan-MGB probe had amplification specificity for transgenic maize MIR162 strain.This method can be used as an effective method to identify and screen transgenic maize MIR162 strains and transformant components,and can be applied for regulating the circulation of transgenic products in the market.