Abstract: Notopterygium incisum jelly was prepared with Notopterygium incisum decoction pieces as the main raw materials, gelatin, agar, stevia glycoside and potassium sorbate as the auxiliary materials. The processing technology and formula of Notopterygium incisum jelly were optimized by single factor and response surface tests. Results showed that the preparation process of Notopterygium incisum concentrate was as follows: 100.00 g Notopterygium incisum decoction slices was soaked with 800 mL pure water for 30 min, and then decocted on high heat for about 40 min, concentrated to Notopterygium incisum concentrate with relative density of 1.0～1.1 at 50 ℃. The best formula of Notopterygium incisum jelly product was: the fixed total volume of Notopterygium incisum concentrate and drinking water 21 mL, the dosage of Notopterygium incisum concentrated solution 19.5 mL, stevioside 0.045 g/mL, potassium sorbate 0.4 mg/mL,and the dosage of mixed gel（the mass ratio of gelatin to agar 0.5∶4.5） 0.1 g/mL. The jelly product prepared under these conditions had a dense porous structure, which endowed the jelly product with certain hardness, cohesiveness, elasticity, chewability, resilience as well as excellent stability, water retention and oxidation resistance, the product had pleasant Notopterygium incisium fragrance and good taste, the content of isoimperatorin was（0.105 8±0.001 6） mg/g, which had certain nutritional value.