Abstract: Using ’Eureka’ blueberries as test material, low-temperature plasma-activated water（PAW） with different treatment times（0, 20, 40, 60 min） were obtained using atmospheric low-temperature plasma-activated deionized water. The blueberry fruits were treated by spraying and soaking methods, and the effects of the two PAW treatment methods on postharvest blueberry sterilization and storage quality at 25 ℃ were compared. According to the results, blueberry fruits treated with PAW spray exhibited a brighter appearance than the fruits treated with PAW soak, and PAW40spray treatment showed the least color difference during the 10 day storage period. The PAW20 soak and spray treatments could better maintain the hardness of blueberry fruits than other PAW treatments. The molds and yeasts growth on the surface of blueberry fruits were more effectively inhibited by the PAW20 and PAW60 spray treatments. The PAW60 treatments had the lowest decay rate, and the PAW spray treatments had a better effect of reducing the decay of blueberries than the soak treatments. The spray treatments could better maintain anthocyanin content of blueberry fruits compared with the soak treatments. In summary, PAW spray treatment is a viable method for storage of postharvest blueberry fruit.