Abstract: In order to prepare elderberry fruit tablets with suitable taste, antioxidant and anti-fatigue effects, with elderberry powder as raw material, the preparation process of elderberry fruit tablets was optimized through response surface test. 1,1-diphenyl-2-trinitrophenyl hydrazine（DPPH） radical scavenging activity, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid（ABTS） cation radical scavenging activity and ferric reducing antioxidant power（FRAP） reducing ability were used as indicators to evaluate its antioxidant capacity. The anti-fatigue ability of mice was evaluated by measuring the swimming time and analyzing the level changes of blood glucose, liver glycogen, muscle glycogen,superoxide dismutase（SOD）, adenosine triphosphate（ATP）, urea nitrogen（BUN）, and lactate（LA）. The results showed that the sensory score and anthocyanins content of elderberry fruit tablets were 90.23 and 1.52 mg/g, respectively,when the addition concentration of elderberry powder, xylitol, and citric acid was 61%, 8% and 5%, respectively.Meanwhile, the DPPH radical, ABTS cation radical scavening capacities, and FRAP values of elderberry fruit tablets were 73.83 μg TE/g, 43.50 μg TE/g and 16.27 μmol Fe（II）/g, respectively, exhibiting good antioxidant capacity. In addition, the intervention of elderberry fruit tablets prolonged the swimming time of mice, increased the levels of blood glucose, liver glycogen and muscle glycogen, and enhanced the SOD and ATP content, as well as reduced the levels of BUN and LA, demonstrating good anti-fatigue effects. Which provides a theoretical basis for the high-value utilization and expanding the application of elderberry fruit.