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冷藏蚕豆鲜荚优势腐败菌的分离与鉴定

Isolation and Identification of Dominant Spoilage Microorganism in Fresh Pods of Broad Bean during Refrigerated Storage

  • 摘要: 为研究蚕豆鲜荚冷藏过程中的优势腐败菌种类及其致腐能力,对10℃下贮藏的蚕豆鲜荚进行腐败菌的分离筛选,通过形态学鉴定及16S rDNA和ITS序列分析,对蚕豆鲜荚的腐败菌进行鉴定;以菌落总数和蚕豆鲜荚感官评分为评价标准,分析优势腐败菌的致腐能力。结果表明:蚕豆鲜荚在10℃下贮藏10 d时的腐败菌为成团泛菌(Pantoea agglomerans)、嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)、韩国假单胞菌(Pseudomonas koreensis)、水生拉恩菌(Rahnella aquatilis)、高山芽孢杆菌(Bacillus altitudinis)、枝胞属菌Cladosporium xanthochromaticum和球孢白僵菌(Beauveria bassiana)。致腐能力由高至低依次为Pseudomonas koreensis>Pantoea agglomerans>Stenotrophomonas maltophilia>Rahnella aquatilis>Bacillus altitudinis>Cladosporium xanthochromaticum>Beauveria bassiana。综合比较,最终确定Pseudomonas koreensis和Pantoea agglomerans为10℃贮藏下蚕豆鲜荚的优势腐败菌。以上研究将为蚕豆鲜荚生物保鲜技术的开发利用提供理论依据。

     

    Abstract: In order to study the dominant spoilage microorganism species in fresh broad bean pods during refrigerated storage and their spoilage capability. The spoilage organism in fresh broad bean pods stored at 10 ℃was isolated and screened, and then identified by morphology observation, 16S rDNA and ITS sequence analysis,and the spoilage capability of the dominant spoilage microorganism was analyzed with total numbers of colony and sensory score as evaluation criterions. The results showed that dominant spoilage microorganism in fresh pods of broad bean stored at 10 ℃ were Pantoea agglomerans, Stenotrophomonas maltophilia, Pseudomonas koreensis,Rahnella aquatilis, Bacillus altitudinis, Cladosporium xanthochromaticum and Beauveria bassiana. The order of spoilage capabilities from high to low was as follows: Pseudomonas koreensis >Pantoea agglomerans >Stenotr-ophomonas maltophilia >Bacillus altitudinis >Rahnella aquatilis >Cladosporium xanthochromaticum >Beauveria bassiana. Comprehensive comparison suggested that Pseudomonas koreensis and Pantoea agglomerans were the dominant spoilage bacteria in fresh broad bean pods stored at 10 ℃. Which will provide a theoretical basis for the development and utilization of biological preservation technology of broad bean pods.

     

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