Abstract: In order to study the dominant spoilage microorganism species in fresh broad bean pods during refrigerated storage and their spoilage capability. The spoilage organism in fresh broad bean pods stored at 10 ℃was isolated and screened, and then identified by morphology observation, 16S rDNA and ITS sequence analysis,and the spoilage capability of the dominant spoilage microorganism was analyzed with total numbers of colony and sensory score as evaluation criterions. The results showed that dominant spoilage microorganism in fresh pods of broad bean stored at 10 ℃ were Pantoea agglomerans, Stenotrophomonas maltophilia, Pseudomonas koreensis,Rahnella aquatilis, Bacillus altitudinis, Cladosporium xanthochromaticum and Beauveria bassiana. The order of spoilage capabilities from high to low was as follows: Pseudomonas koreensis ＞Pantoea agglomerans ＞Stenotr-ophomonas maltophilia ＞Bacillus altitudinis ＞Rahnella aquatilis ＞Cladosporium xanthochromaticum ＞Beauveria bassiana. Comprehensive comparison suggested that Pseudomonas koreensis and Pantoea agglomerans were the dominant spoilage bacteria in fresh broad bean pods stored at 10 ℃. Which will provide a theoretical basis for the development and utilization of biological preservation technology of broad bean pods.