Abstract: The biological organic fertilizer with promoting function was prepared by secondary fermentation of agricultural organic waste composting made with different raw material ratios using the microbial inoculum containing a variety of functional bacteria（Streptomyces alboflavus TD-1, nitrogen fixing bacteria JZ1, inorganic phosphorus decomposing bacteria JWP1, organic phosphorus decomposing bacteria JP12 and potassium decomposing bacteria JK1）and used for planting of cuttage Sedum aizoon L. to explore the effects of the biological organic fertilizer on the yield and quality of the Sedum aizoon L.. The results showed that compared with no-fertilization group and no-secondary fermentation organic fertilizer group, the bio-organic fertilizer after secondary fermentation with different proportions of raw materials significantly improved the yield and quality of Sedum aizoon L.. After 70 days of planting, the growth index of cabbage was better in the N3M bio-organic fertilizer group, with the highest branch number of fibrous root and yield per plant, which was 15.00 and 11.89 g, respectively. The plant height increment reached456.10%, significantly higher than that in the no-fertilization group. Among the quality indexes, the tannin content of Sedum aizoon L. in the N3M treatment group was 2.19 mg·kg^-1, which was 40.5% lower than that in the no-fertilization group, and the astringency of Sedum aizoon L. was significantly reduced, the VC and total flavonoid contents of Sedum aizoon L. were also higher than those in the no-fertilization group and the no-secondary fermentation group. In summary, the application of bio-organic fertilizer could promote the growth of Sedum aizoon L. and improve its quality and yield. Therefore, that biological organic fertilize with appropriate formula can be used for agricultural production as a green and environment-friendly fertilizer.