Abstract: In order to explore the effects of cold storage plate on the defrosting temperature fluctuation, the defrosting process of cold storage with cold storage plate was simulated numerically based on computational fluid dynamics, and the reliability of the CFD model was verified by experiments, then the contours of temperature changes of refrigeration house were obtained during defrosting process. Meanwhile, a small refrigeration storage with two parallel air coolers for continuous defrosting was investigated under four different scenarios（the air cooler frosts with/without cold storage plate, the air cooler does not frost with/without cold storage plate）. Results showed that during defrosting process, the average temperature of cold storage with cold storage plate was lower than that of without cold storage plate through determining the temperatures of fields with different heights sections; during the defrosting of defrost fan, the temperature fluctuation of cold storage was decreased by 47.20% when the cooling fan was frosted; when the refrigeration fan was unfrosted, the temperature fluctuation of cold storage was reduced by 58.95% by the cold storage plate.