Abstract: Through investigating low-temperature vacuum distillation technology of acetic acid fermentation broth,according to the single factor test results, with vacuum degree, distillation temperature and distillation time as factors, yield of distilled vinegar as response value, the production process of distilled vinegar was optimized by response surface analysis. The optimal production conditions were obtained as follows: vacuum degree 0.04 MPa,distillation temperature 52 ℃, and distillation time 15.5 h. Under these conditions, validation test results showed that the yield of distilled vinegar was 75%.