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花色苷稳定性及其微胶囊制备研究

Study on Stability of Anthocyanins and Preparation of Microcapsules

  • 摘要: 采用紫外分光光度法,分析温度、光照、pH及蔗糖质量浓度4个因素对花色苷稳定性的影响;为提高花色苷的稳定性,研究以海藻酸钠为壁材,CaCl2和壳聚糖为固化剂,制备花色苷微胶囊的工艺配方。结果表明,花色苷稳定性降低(以花色苷质量分数低于30%为限)的临界条件分别为:40℃、室内光、pH 3.0、蔗糖质量浓度100 mg/mL,其中温度对其稳定性的影响最大;花色苷微胶囊的最佳制备工艺为:使用针头孔径为0.70 mm的注射器,芯壁比1∶4(V/V),海藻酸钠质量浓度30 mg/mL,CaCl2质量浓度20 mg/mL,壳聚糖质量浓度15 mg/mL,该工艺条件下制备的花色苷微胶囊呈球状,表面光滑,包埋率为91.54%。

     

    Abstract: Ultraviolet spectrophotometry was used to analyze the effects of temperatures, light, pH and sucrose concentrations on the stability of anthocyanins. In order to improve the stability of anthocyanins, the process formula of anthocyanin microcapsules preparation was investigated with sodium alginate as wall material, CaCl2 and chitosan as curing agents. The results showed that the critical conditions for the stability reduction(limit if anthocyanins mass fraction was less than 30%) of anthocyanins were 40 ℃, indoor light, pH value of 3.0, sucrose concentration of 100 mg/mL, and temperatures had the greatest effects on the stability. The best preparation technology of anthocyanin microcapsules was determined as follows: syringe needle aperture of 0.70 mm, core to wall ratio of 1∶4(V/V), sodium alginate mass concentration of 30 mg/mL, CaCl2 mass concentration of 20 mg/mL, chitosan mass concentration of 15 mg/mL. Under these conditions, the prepared anthocyanin microcapsules were spherical and smooth with embedding rate of 91.54%.

     

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