Abstract: In this study,the synergic effects of essential oils fumigation combined with carbon dioxide （CO₂） modified atmosphere packaging on inhibiting the growth of Aspergillus flavus were preliminarily discussed.The antibacterial effects of 0.01μL/cm~3essential oil gas combined with 35%CO₂,50%CO₂ and 65%CO₂ were evaluated by monitoring the growth of A.flavus colonies.The results showed that essential oils fumigation could significantly improve the bacteriostatic effects of CO₂ modified atmosphere packaging alone.When treated for 6 days,the colony diameter of prickly ash essential oil combined with 50%CO₂ modified atmosphere sample was 24%smaller than that of 50%CO₂ air conditioning sample,and the colony diameter of prickly ash essential oil combined with 65%CO₂ modified atmosphere sample was 4%smaller than that of 65%CO₂ air conditioning sample,but the bacteriostatic effects were related to the essential oil compositions and CO₂ concentrations.The antibacterial effects of essential oil combined with high concentrations of CO₂ were higher than that with low concentrations of CO₂.After identification,the main volatile compounds of litsea cubeba essential oil,prickly ash essential oil and lemon essential oil were β-pinene,α-pinene and sabinene The results provided a basis for the development and utilization of natural bacteriostatic agents,the improvement of air conditioning packaging and the extension of storage life of agricultural products.