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秋茄低温胁迫转录组分析及脱落酸信号途径基因挖掘

Transcriptome Analysis and Gene Discovery of Abscisic Acid Signaling Pathway in Kandelia obovata under Low Temperature Stress

    摘要
  • 摘要:
    目的 深入了解秋茄响应低温胁迫的分子机制以及培育秋茄的抗寒新品种。
    方法 以耐寒红树植物‘龙港’秋茄1年生容器苗为实验材料，15 ℃处理12 h为对照组（CK），−5 ℃处理12 h为低温组（LT），采用 Illumina HiSeq测序平台进行转录组测序，挖掘脱落酸信号途径相关基因。
    结果 转录组测序共鉴定到148个转录因子，分属于25个转录因子家族，其中，ERF、NAC、WRKY、bHLH、MYB、bZIP、HB-other和MYB-related等家族所包含的基因数量较多，分别为17、14、12、12、10、9、6和6；差异组共筛选到1 330个差异表达基因（Differentially Expressed Genes, DEGs），其中，698（52.48%）个上调表达，632（47.52%）个下调表达; KEGG通路富集分析发现，DEGs显著富集在植物激素信号转导、苯丙素生物合成、半乳糖代谢、光合作用-天线蛋白和α-亚麻酸代谢等通路中; 脱落酸信号途径相关基因 KoPYL1、KoABF1和KoABF2上调表达，KoPP2C1和 KoABF3下调表达，且这些基因表达情况与qRT-PCR验证结果一致。
    结论 ERF、NAC、WRKY、bHLH、MYB、bZIP、HB-other和MYB-related等家族转录因子对秋茄响应低温胁迫起重要调控作用。植物激素信号转导、苯丙素生物合成、半乳糖代谢、光合作用-天线蛋白和α-亚麻酸代谢等是秋茄响应低温胁迫的重要KEGG通路。脱落酸信号途径中的KoPYL1、KoPP2C1、KoABF1、KoABF2和KoABF3基因可作为后期研究秋茄响应低温胁迫的重要候选基因。

     

    Abstract:
    Objective To understand the molecular mechanism of Kandelia obovata in response to low temperature stress and cultivate new varieties of cold resistance.
    Method Based on the annual container seedlings of 'Longgang' K. obovata, a cold-tolerant mangrove cultiva, the control group ( CK ) was treated at 15 oC for 12 h and the low temperature group ( LT ) was treated at −5 oC for 12 h. Illumina HiSeq sequencing platform was used for transcriptome sequencing, and the genes related to abscisic acid signaling pathway were discovered.
    Result The results showed that a total of 148 transcription factors were identified by transcriptome sequencing, which belonged to 25 transcription factor families. Among them, ERF, NAC, WRKY, bHLH, MYB, bZIP, HB-other and MYB-related families contained more genes, which were 17, 14, 12, 12, 10, 9, 6 and 6, respectively. A total of 1 330 differentially expressed genes ( DEGs ) were screened in the differential group, of which 698 ( 52.48% ) were up-regulated and 632 ( 47.52% ) were down-regulated. KEGG pathway enrichment analysis showed that DEGs were significantly enriched in plant hormone signal transduction, phenylpropanoid biosynthesis, galactose metabolism, photosynthesis-antenna protein, and alpha-Linolenic acid metabolism. Among the abscisic acid signaling pathways, KoPYL1, KoABF1, and KoABF2 were up-regulated and KoPP2C1 and KoABF3 were down-regulated, and the expression of these genes was consistent with the qRT-PCR validation results.
    Conclusion ERF, NAC, WRKY, bHLH, MYB, bZIP, HB-other and MYB-related play an important role in regulating the response of K. obovata to low temperature stress. Phytohormone signal transduction, phenylpropanoid biosynthesis, galactose metabolism, photosynthesis-antenna protein and alpha-Linolenic acid metabolism are important KEGG pathways in K. obovata in response to low temperature stress. KoPYL1, KoPP2C1, KoABF1, KoABF2 and KoABF3 genes in abscisic acid signaling pathway can be used as important candidate genes for future studies on the response of K. obovata to cold stress.

     

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