Abstract: The selection of suitable reference gene is an important prerequisite for successful gene expression analysis by quantitative real-time PCR (qPCR). The authors investigated the expression stability of 12 endogenous house-keeping genes (ACT4, APm, Chc, Gapc, RPL1, RPL2, EF1, EF2, eIF, E3UL, UBQ and UPL2) in 31 samples of Larix kaempferi (Lamb.) Carr. including needles, stems, roots, pollens, zygotic embryos and somatic embryos. The GeNorm and NormFinder algorithms analysis reveals that the APm is the most stable gene among different organs and developmental stages (somatic embryogenesis and seed germination), EF1 and eIF are most stable among stems and needles during plant growth (90 days, 1.5 years, 5 years, 10 years, 25 years and 50 year), respectively. The results would provide optimum internal reference genes for larch gene expression analysis using qPCR.