Abstract: With extracts of blueberry as material, purification of anthocyanin in blueberry by macroporous resins was studied in this paper. The absorption capacity and desorption effect of 12 kinds of macroporous, and the static optimum resin adsorption isotherms for five kinds of macroporous resin which is better on anthocyanin separation effect was investigated. The processing parameters were optimized based on the adsorption and desorption results. The results indicated that XDA-7 resin had best efficacy in separating and purifying the anthocyanins from blueberry. The optimum conditions of adsorption process were pH 3.0, mass concentration 0.94 g/L and flow rate 30 mL/h (2 bed volume). The maximum adsorption capacity is 15.41 g/L wet resin. The optimum elution conditions were room temperature, pH 3.0, velocity of 80% methanol taken as eluent 60 mL/h, and volume of 80% methanol 75 mL. The optimum desorption rate is 92.65%. The anthocyanin produced by this technological process was purplish dark powder after freeze-drying, and the yield of anthocyanin was 70.2%. The anthocyanin's purity increased from 2.20% to 24.54%, and the color value was 121.