Abstract: Using the cotyledon node method and recombinant inbred line population（RIL） 100 families derived from high regeneration rate soybean variety Hefeng 25 and low regeneration rate soybean line L-28, adventitious shoot induction rate, elongation rate, rooting rate and seedling rate were evaluated in order to compare the regeneration rate differences among different soybean genotypes,and simplified genome sequencing technology was used for QTL mapping analysis. The results showed that among the four regeneration traits of soybean RIL population, the induction rate and elongation rate, rooting rate and seedling rate all reached extremely significant difference levels, and all showed continuous unimodal curves, conforming to a normal distribution. Total 10 QTL loci which significantly related to soybean regeneration traits were detected, including two independent QTL loci and three repeatable QTL loci. QTL loci which were significantly related to induction rate, elongation rate, rooting rate and seedling rate were 2, 3, 3, and 2, respectively. The results could lay the foundation for exploring soybean regeneration rules, establishing an efficient regeneration system, and improving soybean genetic transformation efficiency.