Abstract: In order to characterize duck NOD-like receptor protein 3（NLRP3） gene and protein,primers based on duck NLRP3 genome sequence（Gene ID: 101795933） were designed to amplify the CDS sequence of this gene by PCR. After sequencing, a 2 805 bp long gene sequence, encoding 934amino acids that contained PYRIN, NACHT, and LRR structural domains was obtained. The p CAGGSduNLRP3-Flag eukaryotic expression plasmid was then constructed and transfected into DEF. A protein that was approximately 104 ku and expressed diffusely in the cytoplasm at 12 h after transfection was detected by Western-blot and indirect immunofluorescence assay. Further, it was found that the protein could form puncta around the perinuclear region after virus stimulation. These results suggested that the full-length CDS sequence of duck NLRP3 was obtained and the recombinant plasmid pCAGGSduNLRP3-Flag was able to express functional NLRP3 protein after transfection with DEF, which provided a reference for subsequent studies on the function of duck NLRP3 gene and protein.