Abstract: In this study, H9c2 rat cardiomyocytes were used to construct an in vitro hypoxia model, and the effects of Lycium ruthenicum Murry anthocyanins on the proliferation of H9c2 rat cardiomyocytes were investigated by measuring the viability, cell cycle, and m RNA and protein expression of proliferation-related factors CCNT2, CDK9 and FOXP1. The results showed that 25, and50 μg·mL^-1AC induction for 24, 36 and 48 h significantly increased the viability of H9c2 rat cardiomyocytes under both normoxic and hypoxic conditions（P＜0.05）, with the best effect of 50 μg·m L-1addition. In the hypoxic group, the number of cells in the G0/G1 phase was significantly increased（P＜0.05） and the number of cells in the S and G2/M phases were significantly decreased（P＜0.05）; the addition of AC resulted in a significant decrease in the number of cells in G0/G1 phase（P＜0.05） and a significant increase in the number of cells in S and G2/M phases（P＜0.05）. The mRNA and protein expressions of CCNT2, CDK9 and FOXP1 were highly significantly down-regulated under hypoxic conditions（P＜0.01）, and the addition of AC significantly up-regulated the mRNA and protein expression of CCNT2, CDK9 and FOXF1（P＜0.05）. These results suggested that Lycium ruthenicum Murray anthocyanins could protective effect on the proliferative activity of H9c2 rat cardiomyocytes under hypoxia by promoting the expressions of CCNT2, CDK9 and FOXP1-related factors, and promoted H9c2 rats cardiomyocyte cycle progression. It was aimed to lay the foundation for developing feed additives for highland animals in hypoxic production mode to improve their hypoxic adaptability.