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番茄抗晚疫病分子标记应用及种质资源筛选

Application of molecular markers for tomato resistance to late blight and screening of germplasm resources

  • 摘要: 番茄晚疫病是番茄生产过程中对番茄造成严重危害的真菌病害之一,对番茄产量和品质造成较大影响。目前最有效的防治方法仍是选育含有抗晚疫病基因的番茄品种。通过建立高效番茄晚疫病分子标记体系,快速筛选抗晚疫病番茄抗病材料。利用优化的分子标记DM1、R2M1S、Ph-3-GL-R/S、TG328共同筛选得到51份含有抗病基因Ph-3的材料;对筛选出来的抗病材料作DAB和NBT染色。结果表明,21P61、21P70抗病材料叶片中H2O2和O2-积累量高于感病型;qRT-PCR分析明确抗病材料21P70响应抗病基因Ph-3表达;但番茄材料21P61不符合Ph-3抗病模式,推测21P61可能含有其他抗病基因背景。

     

    Abstract: Tomato late blight is one of the most harmful fungal diseases in tomato production,which has great influence on the yield and quality of tomato. At present, the most effective control method is still to breed tomato varieties containing late blight resistance genes. By establishing efficient molecular marker system of tomato late blight, tomato resistant materials against late blight were quickly screened. The optimized molecular markers DM1, R2M1S, PH3-GL-R/S and TG328 were used to select51 materials containing resistance gene Ph-3. The results of DAB and NBT staining showed that the accumulations of H2O2 and O2- in leaves of 21P61 and 21P70 resistant materials were higher than those of susceptible materials. The resistance material 21P70 was identified by qRT-PCR in response to the expression of resistance gene Ph-3. However, tomato material 21P61 was inconsistent with the resistance pattern of Ph-3, which supposed that 21P61 might contain other resistance gene backgrounds.

     

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