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响应面法优化雨生红球藻培养基

Optimization of culture medium of Haematococcus pluvialis by response surface methodology

  • 摘要: 雨生红球藻(Haematococcus pluvialis)是生产高值天然虾青素(Astaxanthin,AST)的优质藻种,优化培养基营养盐组成对提高藻细胞密度和虾青素产量具有重要意义。以雨生红球藻为受试藻株,BG11为基础培养基,作单因素试验筛选NaNO3、MgSO4·7H2O、K2HPO4·3H2O和Na2CO3共4种营养盐最适浓度范围,采用响应面法优化培养基关键营养盐浓度,并作验证试验。结果表明,(1)雨生红球藻相对适宜营养盐NaNO3、MgSO4·7H2O、K2HPO4·3H2O和Na2CO3质量浓度范围分别为1 500~2 000、80~640、20~60和20~80 mg·L-1,对藻细胞密度的影响强弱依次为NaNO3、Na2CO3、K2HPO4·3H2O和MgSO4·7H2O;(2)通过响应面法分析得到上述四种营养盐最佳盐浓度分别为1 556.42、51.43、42.18和358.59 mg·L-1,其中K2HPO4·3H2O和MgSO4·7H2O具有显著交互作用(P<0.05);(3)使用优化后培养基培养雨生红球藻8 d后,藻细胞Fv/Fm与Chl-a浓度分别为0.69和7 268.97μg·L-1,均极显著高于对照组(P<0.01),优化组细胞密度为3.53×10~5mL-1,较对照组显著提高20.48%(P<0.05)。

     

    Abstract: Haematococcus pluvialis is an excellent algae species for production natural astaxanthin. Optimizing the nutrients composition of the culture medium is important to improve the density of algal cells and astaxanthin content. A single-factor test was conducted to screen the optimal concentration range of NaNO3, MgSO4·7H2O, K2HPO4·3H2O and Na2CO3, and the medium was optimized using the response surface methodology and validation tests. The results showed as the followings:(1)The mass concentration of NaNO3, MgSO4 ·7H2O, K2HPO4 ·3H2O and Na2CO3, were in the ranges of 1 500-2 000, 80-640, 20-60 and 20-80 mg·L-1, respectively. The most promoting effects on algal cell density were in the order of NaNO3, Na2CO3, K2HPO4 ·3H2O, MgSO4 ·7H2O.(2) The optimal nutrient concentrations respectively were 1 556.42, 51.43, 42.18, and 358.59 mg·L-1. The interaction between K2HPO4·3H2O and MgSO4 ·7H2O was significant(P<0.05).(3) Cultured H. pluvialis for 8 days in the optimized medium, the Fv/Fm of PS Ⅱ and Chl-a content of the algae cells were 0.69 and 7 268.97 μg·L-1,respectively, which were both highly significant higher than that in the control group(P<0.01), and the cell density was 3.53×10~5m L-1, which was significantly higher than the control by 20.48%(P<0.05).

     

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