Abstract: Phosphorus is an essential nutrient for plant growth, but its utilization efficiency is low,especially in saline-alkali soil. Application of salt-tolerant and phosphate-solubilizing microorganisms is an effective way to improve phosphorus utilization rate in saline-alkali soil. Saline-alkali-tolerant and phosphate-solubilizing bacteria were isolated from the saline-alkali soil of Qiqihaer City, Heilongjiang Province. Fifty-three strains of bacteria were isolated and purified by using the dilution and coating method of PVK medium and LB medium. Combined with chemical determination, a strain P-W13with strong phosphorus solubilizing ability was finally obtained. Through transmission electron microscopy and molecular biology identification, P-W13 was identified as Enterobacter sp. The highest phosphate solubilizing ability of the strain was 89.86 mg·L^-1at 144 h of cultivation. The strain could produce fumaric acid, succinic acid and propionic acid, and the content of propionic acid was the highest. There was a significant correlation between the phosphate-solubilizing ability of the strain and the p H value of fermentation broth and the growth rate of the strain. Strain P-W13 showed strong phosphate solubilization ability even at the concentration of 10% NaCl and pH 10.The contents of IAA and EPS produced by strain P-W13 were 42.10 and 72.55 mg·L^-1, respectively.In addition, the germination rate, shoot length, and root length of seeds treated with strain P-W13fermentation broth were 5.68%, 68.35% and 94.10%, respectively and higher than those of the control. The P-W13 strain of Enterobacter sp. had strong phosphate solubilizing ability, saline-alkali tolerance and plant growth promotion effect, which provided excellent microbial resource for salinealkali soil improvement.