Abstract: Our country has the most abundant genera and species of Pinaceae plant in the world, including ten genera: Pinus, Picea, Cathaya, Pseudotsuga, Larix, Pseudolarix, Cedrus, Tsuga, Keteleeria and Abies. The liposoluble substance in pine needles from Pinaceae plant contain some compound with novel structure and significant bioactivity. In this paper, the domestic and foreign research progress on extraction method, chemical constituents, biological activity, and its application in biomedical and agricultural field of liposoluble substance from pine needles are reviewed. It mainly includes acidic materials and neutral materials. The main extraction solvents were petroleum ether, dichloromethane, and n-hexane. The application of liposoluble substance from pine needles in the development of drugs, green ecological and safe feed additives, plant protection agents, veterinary drugs, and plant growth promoters were also introduced. Suggestions for future study on protection and utilization of pine needles resources from Pinaceae plant, as well as research and development of liposoluble substance from pine needles are proposed.