Abstract: WRKY transcription factor plays an important role in the regulation of plant growth and stress response. A WRKY protein GmWRKY52（NP＿001237726.2）, which may interact with GmNF-YA13, was found by screening the soybean cDNA library after drought treatment by yeast two-hybrid. For identifying any potential interactions between the two, this study cloned GmWRKY52 and carried out bioinformatics analysis, identified the interaction between GmWRKY52 and GmNF-YA13 by yeast two-hybrid. The results showed that the GmWRKY52 gene was 1 163 bp in full length and encoded a protein composed of 265 amino acids. And the multi-sequence alignment and phylogenetic tree analysis showed that GmWRKY52 was closely related to wild soybean GsWRKY69（XP＿028186817.1）. Yeast two-hybrid results showed no interaction between the GmWRKY52 and GmNF-YA13 proteins. The results show that GmWRKY52 and the GmNF-YA13 protein do not interact within yeast cells.