Abstract: GRAS transcription factors play important roles in resisting abiotic stresses in plants. In order to study the response of GRAS genes to drought stress in soybean, and to provide a molecular basis for mining the function and molecular mechanism of soybean GRAS transcription factors in response to drought stress, we analyzed the microarray data of soybean in Gene Expression Omnibus（GEO） database to identify drought responsive GRAS genes. The cis-acting elements in the promoters of the drought responsive GRAS genes were analyzed by PlantCARE. Then we cloned and performed bioinformatics analysis of a GRAS gene, GmGRAS27, which was up-regulated by drought stress in both the vegetative stage（V6） and the full flowering stage（R2）. The promoter of GMGRAS27（Glyma.06 g265500） gene contains both ABRE responsive element and MYB/MYC binding element. Furthermore, the expression of GmGRAS27 under drought, salt and ABA stress was analyzed by using the qRT-PCR method. The results displayed that, in the 117 soybean GRAS genes, 10 genes responded to the drought stress. All of the ten GRAS genes were found to possess at least one stress or hormone responsive element. GmGRAS27 was up-regulated by drought stress in both the vegetative stage（V6） and the full flowering stage（R2）, and it encoded the product GmGRAS27 containing a conserved GRAS domain. The structure of GmGRAS27 was found to be mainly composed of α-helixes and random coils. GmGRAS27 was not only positively regulated by drought stress, but also induced by salt and ABA stress. These results suggested that GmGRAS27 might be an important candidate gene responding to abiotic stresses in soybean.