Abstract: TIFY transcription factors are involved in the regulation of plant growth and development, as well as plant stress response, and play an important role in plant growth regulation. In order to explore the role of TIFY gene family members in the resistance of soybean cyst nematode（Soybean Cyst Nematode, SCN） race 3, in this study, resistant variety Dongnong L-10 and susceptible variety Heinong 37 were treated with SCN3 race stress. Several TIFY transcription factors related to resistance were screened from transcriptome sequencing data, and bioinformatics prediction and analysis were carried out. The results showed that 23 differentially expressed TIFY transcription factors related to race SCN3 were identified and screened from transcriptome data, of which 18 were up-regulated and 5 down-regulated. TIFY gene was expressed in roots, stems and leaves. Under the same stress of soybean cyst nematode, the expression of TIFY in resistant varieties was higher than that in susceptible varieties. There were great differences in physical and chemical properties among different TIFY transcription factors. 23 transcription factors belong to hydrophilic proteins, most of them were acidic isoelectric points. The prediction results of subcellular localization showed that TIFY transcription factors play an important role in nucleus, cytoplasm and mitochondria. In the protein secondary structure, the amino acid sequence was mainly composed of irregular curl, followed by α-helix, β-fold and β-rotation scattered in the protein sequence. Genes were distributed on 10 chromosomes of soybean, there was more serine in the phosphorylation site. There were many cis-acting elements in the promoter that respond to hormone response and stress. There were interaction proteins in the protein encoded by TIFY gene. The results showed that 23 TIFY transcription factors related to race SCN3 could provide an important reference for further understanding the function of TIFY gene in soybean.