Abstract: MYB is an important regulatory gene involved in the synthesis of anthocyanins, with numerous and diverse functions. In order to analyze the function of the homologous genes of AhTc1（MYB family） in soybean and the regulatory gene of black seed coat of peanut, this study cloned the MYB family gene Glyma.18 G261700 based on the sequence information of AhTc1 gene, carried out the analysis of biological information on Glyma.18 G261700, and detected anthocyanin and gene expression in different parts of soybean seedlings with different seed coat colors. The results showed that Glyma.18 G261700 gene was 1 572 bp full length, encoded 189 amino acids, and was belonged to R2 R3-MYB. The protein encoded by AhTc1 was a hydrophobic protein, but the protein encoded by Glyma.18 G261700 was a hydrophilic protein. In the tertiary structure, the tails of Glyma.18 G261700 and AhTc1 were different, and may have different functions in regulating the color of the seed coat. The root anthocyanin content in roots of soybean seedlings with different seed coat colors was lower, while the expression of the Glyma.18 G261700 gene in their roots tended to be higher. The results showed that the high expression of this gene inhibited anthocyanin synthesis in soybean roots at seedling stage, but the regulation of seed coat color should be further explored.