Abstract: In order to evaluate the genetic stability of the target traits of 30% glyphosate water（Monsanto） tolerance in transgenic herbicide tolerant soybean at different generations, we used transgenic soybean ZH10-6 with G2-EPSPS and GAT genes as materials to detect the target protein content in different generations（T₂-T₄）, growth stages and organs of transgenic soybean by enzyme-linked immunosorbent assay（ELISA）.At the same time, we investigated the tolerance of three generations of transgenic soybean to the target herbicide by spraying different doses of glyphosate. The results showed that, the target proteins G2-EPSPS and GAT could be detected stably in all transgenic materials, and the expression levels were basically the same among different generations. The expression of target protein was the highest in leaves and the lowest in seeds. Under the condition of spraying the same dose of glyphosate, the plant height, seedling rate and damage rate of transgenic soybean ZH10-6 in three generations were significantly different to those of non-transgenic control soybean（P＜0.05）, and showed strong tolerance to 4 times medium dose of target herbicide. The results showed that ZH10-6 could be stably inherited in the offspring, which provided data support for the biosafety evaluation of transgenic herbicide tolerant soybean.