Abstract: Genetic improvement of soybean varieties is an important way to achieve high yield, high quality, multi resistance, and wide adaptability. Clarifying the parental sources and pedigree relationships of varieties is of great guiding significance for parental selection, rational combination, and variety breeding. This article conducts genealogical analysis on 31 soybean varieties cultivated by Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences and its cooperative units, which have been approved in recent years, to calculate the genetic contribution values of ancestral parents′ nuclei and cytoplasm. The results indicate that using high-yield and high-quality germplasm resources adapted to the local environment as recipient parents, introducing beneficial molecular modules such as lodging resistance, disease resistance, and drought resistance, improving narrow blood relationships, and enriching genetic basis are the key to the success of soybean breeding in series such as Muyu, providing a theoretical basis for future breeding work.