UV-B处理对大豆异黄酮合成影响及GmUVR8基因克隆与表达分析

张艺; 于存浩; 马晓红; 姚陆铭; 武天龙; 王彪

UV-B处理对大豆异黄酮合成影响及GmUVR8基因克隆与表达分析

1. 上海交通大学农业与生物学院;
2. 东北农业大学农学院

基金项目:

国家自然科学基金(31871645)

详细信息

作者简介:
张艺(1996—),女,硕士研究生,主要从事大豆分子生物学研究。E-mail:gloriazhang@sjtu.edu.cn

通讯作者:
王彪(1973—),男,博士,副教授,主要从事豆类育种和大豆分子生物学研究。E-mail:wangbiao@sjtu.edu.cn

中图分类号: S565.1
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出版历程
- 收稿日期: 2021-06-26
- 刊出日期: 2022-01-19

Analysis of Isoflavone Synthesis Influenced by UV-B Treatment and Cloning of GmUVR8 Gene in Soybean

1. School of Agriculture and Biology, Shanghai Jiaotong University;
2. College of Agriculture, Northeast Agricultural University

摘要

摘要: 为了探究UV-B辐射对大豆异黄酮合成调控的分子机理,本研究以UV-B辐射处理大豆V1期幼苗,采用HPLC和定量PCR方法分别测定处理前后各部位异黄酮含量和基因的表达,并克隆出GmUVR8光受体基因。发现UV-B辐射处理大豆V1期幼苗8 h后,叶中总异黄酮含量提高1.3倍,大豆苷元和染料木素分别提高15.8和16.5倍。大豆根、茎和叶中CHS和IFS基因对UV-B反应的时间和强度存在明显差异,CHS11在处理2 h后表达量增加38.4倍,IFS1和IFS2基因的最高表达量分别比处理前增加4.7和18.3倍。克隆出了大豆中编码UVR8光受体的GmUVR8a、GmUVR8b和GmUVR8c基因,其氨基酸序列与拟南芥AtUVR8的同源度为74%;GmUVR8a、GmUVR8b、GmUVR8c基因在叶中表达存在显著差异,以GmUVR8b表达量最高。结果表明在大豆中UV-B辐射可能通过多种UVR8光受体调控苯基丙酸类途径关键酶基因的表达,进而影响异黄酮的合成。
- 大豆 /
- UV-B /
- UVR8光受体 /
- 异黄酮 /
- 基因表达 /
- 基因克隆
Abstract: In order to explore molecular mechanism of soybean isoflavone synthesis influenced by UV-B radiation, isoflavone accumulation and gene expression levels in different parts of V1 stage, seedlings under UV-B treatment were investigated by HPLC and quantitative real-time PCR respectively, and genes encoding UV RESISTANCE LOCUS 8（UVR8） photoreceptor were also cloned. The accumulation of total isoflavones in leaves of soybean V1 stage was increased by 1.3 times under 8 h UV-B radiation, and daidzein, genistein improved by 15.8 and 16.5 times respectively. The transcripts of CHS and IFS exhibited obvious difference response to time after UV-B stress, and CHS11 was increased 38.4 times after 2 h treatment, and the highest expression levels of IFS1 and IFS2 increased by 4.7 and 18.3 times respectively. The coding sequences of UVR8 photoreceptor GmUVR8 a, GmUVR8 b and GmUVR8 c were cloned from soybean, the encoding protein had 74% homology in amino acids with AtUVR8, their expression levels in leaves showed significant difference, and GmUVR8 b displayed the highest transcripts among those genes. The results indicated that UV-B radiation might regulate synthesis of soybean isoflavones through multiple UVR8 light receptors which affect the expression of key enzymes in phenylpropanoid pathway.
- soybean /
- UV-B /
- UVR8 photoreceptor /
- isoflavones /
- gene expression /
- gene clone

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