Abstract: To understand the inheritance pattern of soluble sugar content for soybean fresh seeds, we applied combined analysis method of mixed quantitative major-genes plus polygenes inheritance model to investigate inheritance of soluble sugar content in Mindou 7, Fudou 234, and the F₁and F₂ populations of Mindou 7×Fudou 234. The results showed that the genetic model 2 MG-EA, incorporating two pairs of equal additive major genes, was the best-fitting genetic model for soluble sugar content of soybean fresh seeds. The additive effects came from the female parent Mindou 7. The heritability of major genes in the F₂ population conferring soluble sugar content was 72.86%. Thus, in the process of vegetable soybean breeding, selected parents should be with relatively high soluble sugar content, and the directional selection of soluble sugar content should be carried out in the early generation.