Abstract: Salt stress is one of the important environmental factors affecting the growth, development and yield of soybean. NAC family transcription factors are important genes in plant response to salt stress, but their functions have not been fully elucidated in soybean. To identity new genes that control salt tolerance in soybean, in this study, the whole genome analysis, cluster analysis and transcriptome data of soybean plants under salt stress were first used to identify NAC genes induced by salt stress. Secondly, the haplotype, salt tolerance index and nucleic acid polymorphism of NAC genes induced by salt stress in roots were analyzed. We identified 182 NAC genes in the soybean genome and divided them to 7 subfamilies. We found that the expression of 18 NAC genes were significantly up-regulated under salt stress, among which eight NAC genes were expressed in roots, which might be related to response to salt stress. An important gene NAC23 that may respond to salt stress was identified. Our results also showed that the Hap1 haplotype of NAC23 may promote the tolerance of cultivated soybean to salt stress, and this gene was subjected to strong artificial selection. These results provide new insights for the comprehensive analysis of the relationship between NAC gene and soybean salt tolerance, and provide gene resources and new ideas for breeding new varieties of highly resistant crops.