Abstract: Triacylglycerol lipase（TGL） is the main enzyme of triglyceride catabolism. TGLs can hydrolyze long-chain fatty acid triglycerides involved in plant growth, development and abiotic stress response. In order to study the TGL gene family members in soybean and their response to high salt stress, this study identified GmTGL genes in soybean, and analyzed the physicochemical properties, systematic evolution, gene structure, conserved domain, chromosome location and tissue expression pattern based on bioinformatics methods. We analyzed the differences of gene expression under salt stress based on the RNA-seq database and the fluorescence quantitative PCR method. The results showed that a total of 15 GmTGL genes were identified from soybean, their encoding amino acid sequences ranged from 392 to 701 aa, and the GmTGL genes was unevenly distributed on 11 chromosomes. These GmTGLs all shared a common conserved domain Abhydro_lipase（PF04083）. The promoter analysis showed that the promoter of GmTGLs contained cis-responsive elements, hormone responsive elements, and stress responsive elements such as ARE, LTR, TC-rich Repeats and MBS. GmTGL3 and GmTGL14 were expressed in all soybean tissues, and the expressions of GmTGL3, GmTGL14, GmTGL2 and GmTGL6 were up-regulated in response to salt stress. GmTGL2 and GmTGL3 genes with the most obvious up-regulated expression under salt stress were selected for semi-quantitative analysis, which was further verified that TGL gene family could participate in the response process of soybean to salt stress.