Abstract: Nattō is a highly nutritious fermented soy product and a popular dietary supplement. The small-sized seed with strong water absorption, good cooking characteristics and uniform seed shape is suitable for nattō processing. In order to develop a new variety suitable for nattō processing, made a positive contribution to the development of nattō health industry, and provided reference for the innovation of soybean germplasm, a new small seed soybean variety Zhonglong Xiaolidou 3 suitable for nattō processing was bred by sexual hybridization with male parent Longpin 8807 which was a high protein, and excellent germplasm and the female parent Zhonglong Xiaolidou 1. Its 100-seed weight was about 8.1 g, the protein content was 42.39%, and the fat content was 18.38%. The variety had medium resistance to gray spot, the seedling-mature fertility time were about 116 d; the required active accumulated temperature of ≥10 ℃ was 2 400 ℃ and the variety was suitable for planting in mid-early maturing spring soybean producing area of northeast China. The average yield of the regional trial was 2 690.0 kg·ha^-1, which was 8.3% higher than that of the control variety Zhonglong Xiaolidou 1. The test showed that Zhonglong Xiaolidou 3 could meet the special requirements of nattō for production materials, and is an ideal special variety for nattō processing.