Abstract: Wandou 39 is a new soybean variety bred by the Crop Institute of Anhui Academy of Agricultural Sciences through sexual hybridization with national approval variety Hedou No.3 as female parent and high protein variety Jidou 12 as male parent. From 2016 to 2017, Wandou 39 participated in the national southern Huang-Huai-Hai soybean variety trial regional test, with an average yield of 2 820.38 kg·ha^-1, increased by 3.88% compared with Zhonghuang 13（CK）. In 2018, it participated in the national southern Huang-Huai-Hai soybean variety trial production test, with an average yield of 2 836.50 kg·ha^-1, 4.76% higher than that of the CK. The test results showed that the protein content of Wandou 39 was 45.81% and fat content was 18.56%. And it was resistant to both SC3 and SC7 strains. In 2019, it was approved by the National Crop Variety Approval Committee with the approval number of 20190017. Its main characteristics are high quality, high yield and strong stress resistance. It is suitable for being planted in areas including southwestern Shandong, central and southern Henan, the north of Huaihe river in Jiangsu and Anhui.