Abstract: In order to identify the relationship between soybean nuclear factor YA（NF-YA） and abiotic stress, we detected the expression levels of GmNF-YA7 and GmNF-YA8 in soybean under drought stress, cloned the GmNF-YA7, constructed the plant expression vector, and obtained genetically engineered strain in this reaserch. qRT-PCR results showed that both expression levels of GmNF-YA7 and GmNF-YA8 could be induced by drought stress, and the response of GmNF-YA7 to drought stress was more obvious. GmNF-YA7 was cloned from soybean, it was located on chromosome 8 of soybean and encoded a protein containing 336 amino acids. The predicted molecular weight of GmNF-YA7 protein was 37.06 kDa and the pI was 6.11. The amino acid sequence of GmNF-YA7 protein contained a conserved CBF domain. Subcellular localization prediction results showed that GmNF-YA7 protein was localized in the nucleus. Phylogenetic analysis showed that GmNF-YA7 protein was closely related to Arabidopsis AtNF-YA1 protein. GmNF-YA7 was constructed into plant expression vector pRI101 with restriction endonucleases Nde I and Sal I and transformed into Agrobacterium EHA105. This study showed that the GmNF-YA7 gene was up regulated by drought, and we constructed plant expression vector with GmNF-YA7 and genetically obtained engineered Agrobacterium strain with this vector.