Abstract: This study examined the segregation distortion during the process of constructing wild soybean and cultivated soybean population, to explore the segregation distortion regions（SDRs） and candidate genes, and to shed some light on understanding the mechanism of segregation distortion in soybean. The wild variety ‘Changling wild soybean’ was used as the male parent and the landrace variety ‘Yiqianli’ was used as the female parent to develop a hybrid group, resulting in 200 recombinant inbred lines（RIL）. SLAF-seq was used for sequencing analysis and constructing a high-density genetic map. 4 564 SNP markers were obtained and reliably identified for this population. Through segregation distortion analysis, 648 markers were found to have genetic distortion（P＜0.05）, accounting for 14.20% of the total markers. 22 SDRs were found, which were distributed across 9 different chromosomes. In SDRs, 8 extreme SDRs regions（ESDRs） were found, distributed on 5 different chromosomes, of which 3 ESDRs were biased towards the wild type of the male parent, and 5 ESDRs were biased towards the cultured type of the female parent. Using gene function annotation and genome-wide resequencing data, combined with the ESDR region, the affecting embryonic development（Glyma.01G051400） and female gametophyte development（Glyma.16G072700） genes were identified as candidate genes in ESDR1-1 and ESDR16-1, respectively. This study provides a reliable basis for locating the segregation distortion genes in the future, and lays the foundation for elucidating the segregation distortion.