Abstract: Qiandou 13 was a new soybean variety with high yield and high resistance to soybean mosaic virus which was bred by Guizhou Oil Research Institute by mutiple hybridization among landraces of Liuzhiliuyuehuang, Dafangfengdingdou, Longlidabaishuidou, Nayongguangkedou and Zhenyuandahuangdou. The average yield of two years from 2017 to 2018 of Qiandou 13 was 2 711.26 kg·ha^-1 which was 5.50% higher than that of the control Qiandou 7 in Guizhou provincial regional trial. The yield was 2 724.15 kg·ha^-1 which was 10.31% higher than that of Qiandou 7 in production test in 2018. The soybean variety showed high resistance to soybean mosaic virus by artificial inoculation with SC3 and SC7 strains. And the protein and the fat content were 44.09% and 19.21%, respectively. The agronomic traits of Qiandou 13 were as follows: Growth period 116.90 d, plant height 56.50 cm, 12.30 main stem nodes, 2.30 branches, 35.10 pods per plant, 52.60 seeds per plant, 100-seed weight 23.90 g, purple flower, gray hair, semi-determinate podding habit, compact plant type, oval leaves, yellow seed coat, brown seed umbilicus. Qiandou 13 was suitable for spring sowing and planting in Guizhou Province which was approved by Guizhou Province with the approval number of Qianshendou 20200002 in 2020.