Effects of Melatonin on Soybean Seeds Development at Seed Filling Stage Under Drought Stress
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摘要: 为探究外源褪黑素对干旱胁迫下鼓粒期大豆籽粒发育的影响，本试验以干旱敏感型大豆品种绥农26和耐旱品种抗线9号为试验材料，采用盆栽土壤称重控水法设置干旱胁迫，于R5期进行褪黑素叶面喷施处理，褪黑素浓度为100μmol·L-1,处理后5,10,15 d进行取样，针对外源褪黑素对干旱胁迫下大豆籽粒发育的影响进行研究。结果表明：在干旱胁迫下喷施外源褪黑素处理可以显著提高干旱胁迫下大豆糖类物质含量，碳代谢关键酶活性，有利于碳代谢水平的提高；同时，外源褪黑素处理显著增加了干旱胁迫下大豆的氮代谢关键酶（NR、GS、GOGAT和GDH）的活性，促进植株氮素积累量提高，有利于氮素同化和转运。综上所述，外源褪黑素处理通过调节干旱胁迫下大豆碳氮代谢关键酶活性、糖类物质含量、氮素积累量促进鼓粒期大豆籽粒发育，最终提升干旱胁迫下大豆的产量。Abstract: In order to explore the effect of exogenous melatonin on soybean seeds development under drought stress, the drought-sensitive soybean variety Suinong 26 and the drought-tolerant variety Kangxian 9 were used as test materials, by potting method, under drought stress, melatonin foliar spraying was carried out at R5 stage, and the melatonin concentration was 100 μmol·L-1, samples were taken after 5, 10 and 15 days of treatment to study the effect of exogenous melatonin on soybean grain development under drought stress was studied. The results showed that exogenous melatonin treatment could improve the activities of soybean sugars, starches, and key enzymes for carbon metabolism under drought stress, and was beneficial to improve the level of carbon metabolism. At the same time, exogenous melatonin treatment significantly increased the activities of key enzymes（NR, GS, GOGAT and GDH） of soybean under drought stress, promoted the accumulation of nitrogen in plants, and was conducive to nitrogen assimilation and transportation. In conclusion, exogenous melatonin treatment improved soybean drought tolerance by regulating the activities of key enzymes in soybean carbon and nitrogen metabolism under drought stress, and ultimately increased soybean yield under drought stress.
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