Abstract: In order to explore the efficient cultivation technology of high protein soybean variety Mudou 15,three factors including sowing date, density and fertilizer application were set to study the effects of these three factors on the main agronomic and photosynthetic characteristics of Mudou 15. The results showed that under the conditions of different cultivation factors, the yield of Mudou 15 varied from 1 958.97 to 2 851.28 kg · ha^-1, with the maximum variation of 45.55%. The average variation range of protein content was 42.19%-45.06%, and the maximum variation range was 6.80%. Sowing date, planting density and fertilizer application amount all had influence on net photosynthetic rate, among which sowing date and fertilizer application amount had little effect on net photosynthetic rate, planting density had more effect on photosynthesis, and the net photosynthetic rate at low planting density was higher. At the same time, high planting density could increase intercellular CO₂ concentration, transpiration rate and chlorophyll content. After screening, the cultivation plan with the highest yield was sown on April 30, with planting density of 300 thousand plants· ha^-1 and 350 kg · ha^-1 for fertilizer application. The cultivation plan with the highest protein content was sown on May 7, with planting density of 300 thousand plants · ha^-1 and 400 kg· ha^-1 for fertilizer application.