Abstract: Nitrate transporters（NRTs） play an important role in NO₃^- uptake or transporters in plant roots. In order to study the function of soybean NRTs genes in response to salt stress, eight differentially expressed GmNRTs genes that respond to salt stress were identified from the transcriptome data of salt stress treatment. The promoters of these GmNRTs genes contain multiple plant stress or plant hormones response elements, suggesting that these genes might be involved in abiotic stress or plant hormone regulation of salt stress response of soybean. At the same time, we cloned GmNRT1.5A from soybean roots, which was the most up-regulated gene under salt stress treatment. The GmNRT1.5A CDs of 1 794 bp in length, encoded a 597 amino acid, with molecular weight of 66.78 kD and isoelectric point（pI） of 5.85. Phylogenetic analysis revealed that GmNRT1.5A had a close relationship with AtNRT1.5 and ZmNRT2.5, contained twelve transmembrane domains and typical NRT1s conserved domains, and the secondary structure of GmNRT1.5A was found to be composed of α-helix and random coils. Real-time quantitative PCR analysis showed that GmNRT1.5A was mainly expressed in seeds and roots. Salt stress and drought stress significantly induced the expression of GmNRT1.5A gene in root. In addition, the expression of GmNRT1.5A was significantly induced by ABA and ACC, while GA₃ treatment significantly inhibited the expression of GmNRT1.5A. This study showed that GmNRT1.5A played an important role in response to salt stress, which provides a new idea for exploring the function of NRTs genes in soybean response to stress, and also lays a foundation for in-depth research on the function of GmNRT1.5A gene in soybean.