Abstract: Sucrose is the main form of plant sugar source and storage transportation, and is an important energy substance in plant growth. In this study, the 196 natural populations consisting of domestic and foreign varieties that had been re-sequenced were used as test materials, and sucrose content of seedling stage in leaves in this population was measured, and a genome wide association analysis was performed with a mixed linear mode. The results showed that 5 significantly related SNP loci were screened according to the threshold, which were located on chromosomes 15 and 20, and 27 related genes were searched in the interval of 100 kb upstream and 100 kb downstream of the SNP loci. The 5 candidate genes that might be related to sucrose content in soybean leaves were screened by GO functional enrichment analysis, KEGG metabolic pathway enrichment analysis and gene function annotation. Three genes related to sucrose content in soybean leaves, including Glyma.15G023800, Glyma.15G024000 and Glyma.15G024600, were identified by relative expression analysis. The results of this study provide a theoretical reference to investigate the genetic mechanism of sucrose content in soybean leaves.