Abstract: In order to study the preparation method and anticancer activity of genistein-zinc and genistein-copper metal complexes, genistein was used as a ligand, and then it was combined with zinc and copper to synthesize genistein-zinc and genistein-copper metal complexes. Metal complexes were characterized by IR, UV and thermogravimetric analysis. The inhibitory effects of genistein and its zinc and copper complexes on the proliferation of human non-small cell lung cancer cell line A549, human breast cancer cell MCF-7 and human umbilical vein endothelial cell HUVEC were investigated by MTT method. The results showed that genistein-zinc and genistein-copper complexes were successfully prepared, and the MTT assay results showed that genistein-zinc and genistein-copper complexes had inhibitory effects on the proliferation of MCF-7 and A549 cells, and the inhibitory effects were higher than genistein, however, genistein-zinc and genistein-copper complexes had no obvious inhibitory effects on the proliferation of HUVEC cells.