Abstract: In order to reveal the role of GmOPRs, a member of soybean 12-oxo-phytodienoic acid reductase（OPR） family in soybean growth and development under abiotic stress, we identified and analyzed the whole genome of soybean OPR gene family with bioinformatics method. The results showed that there were twelve OPR genes distributed on 8 chromosomes in soybean genome. The phylogenetic analysis, gene structure analysis and conserved motifs analysis showed that GmOPRs could be divided into two subgroups, containing 4-5 exons and 3-5 introns respectively. The conserved motifs of GmOPR proteins were similar in distribution. Colinear analysis identified colinear relationship among four pairs of genes, all of which were fragment replication. The promoter region of GmOPRs contained cis-acting elements responding to abiotic stress such as anaerobic, drought and low temperature, JA, ABA and other hormones. The expression patterns of GmOPRs were different in different soybean varieties and tissues. The expression of GmOPR1, GmOPR4 and GmOPR9 decreased under drought stress, while the expression of GmOPR7, GmOPR8, GmOPR11 and GmOPR12 increased over time under salt stress, suggesting that the GmOPRs responded to drought and salt stress through multiple expression patterns. GmOPRs were conserved in phylogenetic evolution and gene structure, and the amplification of the gene families was mainly attributed to fragment repetition. GmOPRs were affected by abiotic stresses such as drought and salt stress, and showed different expression patterns. The results suggest that GmOPR gene family may play an important role in abiotic stress responding.