Abstract: In order to screen suitable rhizobia strains for application under saline alkali soil conditions, this study selected soil samples from Anda City and used soybean varieties Mancangjin and Suinong 53 as hosts to isolate and purify rhizobia. Two strains with typical rhizobia phenotype characteristics were screened. Subsequently, 16S rDNA sequencing analysis was performed on these strains, and two rhizobia strains were successfully isolated, named an28 and an44. Perform nodulation experiments on each captured host to determine the nodulation characteristics of an28 and an44. The results of antibiotic resistance identification showed that an44 was resistant to erythromycin（ERY） at a concentration of 20 μg·mL^-1 and carbenicillin（CAR） at a concentration of 100 μg·mL^-1, while an28 was resistant to ERY at a concentration of 50 μg·mL^-1. The salt tolerance identification results showed that an28 can grow normally on TY solid culture medium with a NaCl concentration of 600 mmol·L^-1; And an44 can only grow at a maximum NaCl concentration of 200 mmol·L^-1. The results of alkali resistance identification indicate that an28 can grow normally on TY solid culture medium at pH11.9, while the alkaline growth environment of an44 can only reach pH9.9. Then, two strains of rhizobia were inoculated on eight salt alkali tolerant soybean germplasms（Qingte 4, Tiefeng 8, Niuyanjing, Heizhenzhu, Qinong 5, Hefeng 50, Heihe 49, and Heinong 531） and two salt alkali tolerant soybean varieties（Jinnong 5 and Shuangbao 8） for nodulation identification. The results showed that the range of nodulation numbers for inoculating rhizobia an28 and an44 on 10 soybean germplasms was 2.00-8.67 and 5.67-35.67 per plant, respectively.