Abstract: In order to analyze the evolution of GmTOEs and its regulation on flowering function in soybean, to provide the basis for functional analysis of GmTOEs and research on latitude adaptability of soybean, we used bioinformatics methods to perform cluster analysis, sequence characterization analysis, chromosomal segment collinearity analysis and tissue specific expression analysis of GmTOEs, predicted AP2 binding sites in the promoter regions of important flowering genes and verified flowering times in different haplotypes of soybean. The results showed that 12 GmTOEs were retrieved from PlantTFDB database, and GmTOE6 b（Glyma.02 G087400） was a newly discovered TOE gene in soybean. GmTOE6 a and GmTOE6 b had only one AP2 domain, while the other GmTOEs had two AP2 domains. Six GmTOEs were clustered into the same group as the Arabidopsis TOE1, two were clustered with Arabidopsis TOE2, four were clustered with Arabidopsis TOE3 and AP2. Each GmTOEs had one miR172 target site, and the target site sequence was highly consistent with AtTOEs. Chromosome segment collinearity analysis showed that GmTOEs could be divided into three categories according to their origin, and six of them were generated with the replication of soybean genome. Four of them originated before the speciation of soybean and shared a common ancestor with AtTOEs. Two of them originated before the speciation of soybean and had no common ancestor with AtTOEs. The important flowering regulation genes GmFT2 a and GmFT5 a have multiple AP2 binding sites in their promoter, and both GmTOE4 b and GmTOE5 b can affect the flowering time of soybean. The results indicated that the 12 GmTOEs were the most likely to be the target of miR172. Although the amino acid composition of the protein encoded by them was very similar, their evolutionary rules and tissue-specific expression were different, and they may have functional differentiation during the evolutionary process. GmTOE4 b and GmTOE5 b may regulate the transcription of GmFT2 a and GmFT5 a by binding to their cis acting elements on the promoters, to regulate flowering.