Abstract: The soybean Glyma03g263000 gene encodes a RING-H2 zinc finger protein, its expression level is affected by soybean cyst nematode infection. In order to provide references for further clarifying its function in nematode resistance, this study performed a bioinformatic analysis and cloned CDS region of the gene from a disease-resistant soybean variety by PCR method. To obtain the purified target protein, prokaryotic expression vector pET30a-Glyma03g263000 was constructed and transformed into E. coli Rosetta（DE3） strain, we set different bacterial concentration, IPTG concentrations, temperatures and inducing times, and detected the target protein by SDS-PAGE electrophoresis to analyze the conditions for inducing the expression of this protein. Bioinformatic analysis showed that the gene encodes 260 amino acids with a molecular mass of 27.88 kDa and a RING conserved domain in the 102-145 amino acid region. Through the analysis of induced expression conditions, it was found that temperature was the main factor affecting protein expression. Glyma03g263000 protein is a water-insoluble inclusion body protein, which can be obtained after treatment with reagents such as denaturation and cleavage. The results of this study laid a theoretical and material foundation for the functional analysis of Glyma03g263000 protein.