Abstract: In order to cultivate the soybean varieties resistant to soybean mosaic virus SC7 strain, which are the main soybean mosaic virus strains in the soybean producing areas of the Huang-huai-hai and Yangtze River basins, the Fuyang Academy of Agricultural Sciences/Anhui Branch of the National Soybean Improvement Center used Zheng 97196 as the female parent and Kefeng 29 as the male parent, a new summer soybean variety Fudou 169 with resistance to mosaic virus SC7 was bred by hybridization and MAS（molecular marker-assisted selection）. The average crude protein content of the two-year trial was 40.96%, and the average crude fat content was 19.4%. The identification results of resistance gene-linked molecular marker BARCSOYSSR＿02＿0631 and artificial inoculation showed that the variety showed resistance to soybean mosaic virus SC3 and SC7 strains. Fudou 169 showed disease resistance to 8 isolates of Phytophthora sojae. The production test yield in 2019 was 2 668.05 kg·ha^-1, and an increase of 9.43% compared with the Zhonghuang 13. In 2020, it was approved by the Anhui Provincial Crop Variety Approval Committee, and the approval number was Wanshendou 20200001. The application of MAS technology has realized the early progeny selection and identification of resistant to mosaic virus SC7 in soybean lines, which not only breaks through the limitations of time and space conditions, but also avoids the transmission of mosaic virus in traditional methods of inoculation and identification, reducing the risk of infection, cuts down on the follow-up and shortens the breeding cycle of disease-resistant and high-yield soybean varieties.