高级检索+

不同程度盐胁迫下大豆萌发期耐盐性鉴定

Salt Tolerance Identification of Soybean at Germination Stage Under Different Salt Stress Degrees

  • 摘要: 为了明确不同程度盐胁迫下大豆萌发期耐盐性鉴定的评价指标,以62份大豆品种为试验材料,在萌发期进行75 mmol·L-1 NaCl中度盐胁迫(S)和150 mmol·L-1 NaCl重度盐胁迫(SS)处理,调查并分析胚根长、干鲜重、发芽率等16项指标,以确定大豆萌发期耐盐性鉴定的适宜筛选浓度,筛选耐盐性大豆品种。结果表明:萌发期各指标耐盐系数之间存在显著或极显著正相关,根长等13个指标与子叶干重、平均发芽时间和平均发芽速度等存在显著或极显著负相关。16项指标的耐盐系数可简化得到4个主成分,在S与SS处理中的累计贡献率分别为88.243%和80.366%。62份材料在两种程度的盐胁迫下均被分为5类,S条件下筛选出龙垦310、黑科68、龙垦330、龙垦306、龙垦333、黑科69和龙垦3002共7个耐盐品种,SS条件下筛选出1个耐盐品种为黑科57。通过建立大豆萌发期耐盐性评价的回归方程,确定了在S条件下的一级评价指标6项,二级评价指标3项;SS条件下的一级评价指标5项,二级评价指标1项。S处理能有效区分出不同大豆品种的耐盐性,也更为接近生产中可能遇到的盐害程度,可作为大豆萌发期耐盐性鉴定的适宜筛选浓度。

     

    Abstract: In order to clarify the evaluation indicators for salt tolerance identification of soybean during germination under different degrees of salt stress, 62 soybean varieties were used as experimental materials in this experiment. During germination, moderate salt stress(S) of 75 mmol·L-1 NaCl and severe salt stress(SS) of 150 mmol·L-1 NaCl were conducted. Sixteen indicators, such as radicle length, dry and fresh weight, and germination rate, were investigated and analyzed to determine the appropriate screening concentration for salt tolerance identification of soybean during germination and screening salt tolerant soybean varieties. The results showed that there was a significant or extremely significant positive correlation between the salt tolerance coefficients of each index at the germination stage, and thirteen indexes such as root length were significantly or extremely significant negatively correlated with cotyledon dry weight, average germination time and average germination speed. The salt tolerance coefficients of the sixteen indexes could be simplified to obtain four principal components, and the cumulative contribution rates in S and SS were 88.243% and 80.366%, respectively. The 62 materials were divided into five categories under two degrees of salt stress, and seven salt-tolerant varieties were screened out under S conditions, including Longken 310, Heike 68, Longken 330, Longken 306, Longken 333, Heike 69 and Longken 3002, and one salt-tolerant variety of Heike 57 were screened under SS conditions. By establishing the regression equation for the evaluation of salt tolerance in the germination period of soybean, six primary evaluation indexes and three secondary evaluation indexes were determined under S condition. There were five first-level evaluation indicators and one second-level evaluation index under SS condition. S treatment could effectively distinguish the salt tolerance of different soybean varieties, and was closer to the degree of salt damage that might be encountered in production, which could be used as a suitable screening concentration for the identification of salt tolerance in the germination stage of soybean.

     

/

返回文章
返回