Abstract:
In order to clarify the evaluation indicators for salt tolerance identification of soybean during germination under different degrees of salt stress, 62 soybean varieties were used as experimental materials in this experiment. During germination, moderate salt stress(S) of 75 mmol·L
-1 NaCl and severe salt stress(SS) of 150 mmol·L
-1 NaCl were conducted. Sixteen indicators, such as radicle length, dry and fresh weight, and germination rate, were investigated and analyzed to determine the appropriate screening concentration for salt tolerance identification of soybean during germination and screening salt tolerant soybean varieties. The results showed that there was a significant or extremely significant positive correlation between the salt tolerance coefficients of each index at the germination stage, and thirteen indexes such as root length were significantly or extremely significant negatively correlated with cotyledon dry weight, average germination time and average germination speed. The salt tolerance coefficients of the sixteen indexes could be simplified to obtain four principal components, and the cumulative contribution rates in S and SS were 88.243% and 80.366%, respectively. The 62 materials were divided into five categories under two degrees of salt stress, and seven salt-tolerant varieties were screened out under S conditions, including Longken 310, Heike 68, Longken 330, Longken 306, Longken 333, Heike 69 and Longken 3002, and one salt-tolerant variety of Heike 57 were screened under SS conditions. By establishing the regression equation for the evaluation of salt tolerance in the germination period of soybean, six primary evaluation indexes and three secondary evaluation indexes were determined under S condition. There were five first-level evaluation indicators and one second-level evaluation index under SS condition. S treatment could effectively distinguish the salt tolerance of different soybean varieties, and was closer to the degree of salt damage that might be encountered in production, which could be used as a suitable screening concentration for the identification of salt tolerance in the germination stage of soybean.