Abstract: In order to select the exogenous reagents that can effectively alleviate the injury of vegetable soybean, and clarify its regulatory effect on vegetable soybean under low temperature stress, the vegetable soybean variety Liaoxian 1 was used as the test variety, and compared the effects of gibberellin, ethephon, ferulic acid γ-aminobutyric acid, chitosan and chlorogenic acid on antioxidant system, osmoregulation substances and nitrogen metabolism of vegetable soybean under low temperature stress. The results showed that exogenous GA₃ and GABA treatment significantly increased the antioxidant enzyme activities, especially the activities of SOD and CAT, significantly increased the contents of ASA and GSH, significantly reduced the content of superoxide anion（O^-₂） and alleviated the oxidative damage of vegetable soybean under low temperature stress. In addition, exogenous GA₃ and GABA treatment significantly increased the soluble sugar and sucrose content of vegetable soybean and enhanced its cell water holding capacity. At the same time, it significantly improved the activities of nitrogen metabolism enzymes in vegetable soybean, especially the activities of nitrate reductase（NR）, glutamate synthase（GOGAT） and glutamate dehydrogenase（GDH）, promoted the nitrogen metabolism of vegetable soybean and enhanced the tolerance to low temperature stress. It can be seen that exogenous GA₃ and GABA treatment can alleviate the cold tolerance of vegetable soybean by regulating antioxidant, osmotic regulatory substances and nitrogen metabolism.